Research Fellow in Bionanotechnology
- University of Leeds
- Location: Leeds, United Kingdom
- Job Number: 7106010 (Ref #: FBSAS1055)
- Posting Date: Jul 21, 2022
Are you an ambitious researcher looking for your next challenge? Do you have a proven track record in bionanotechnology? Do you have an established background in proteomics or structural mass spectrometry?
We are seeking a highly motivated and talented individual interested in undertaking a challenging and exciting 3 year postdoctoral project in the laboratory of Professor Neil Ranson, Dr Paolo Actis and Professor Frank Sobott.
You will be leading efforts in an exciting new Multi-modal Multi-scale project funded by the Medical Research Council (MRC) with the goal of unravelling the tissue-specific geography of protein aggregation in human disease. In collaboration with the University of Newcastle, the overarching goal is to study aggregation pathways of the peptide hormone hIAPP (human islet amyloid polypeptide) in the pancreas linked to Type 2 diabetes mellitus (T2DM).
In this exciting and ground-breaking project, you will use a novel sampling technique based on nanopipettes to extract hIAPP aggregates and associated soluble material directly from thin sections of human pancreas and from cultured human islets in order to characterise aggregation as a function of pancreatic geography. You will also generate and investigate hIAPP aggregates in vitro to create reference datasets for this study, and to be able to challenge tissue by injecting samples into them. You will employ various mass spectrometry (MS) methods, including proteomics and structural MS approaches (e.g., native & limited digest-MS, crosslinking and covalent labelling by Fast Photochemical Oxidation of Proteins). These will be the main readout for protein sequence variations (e.g., PTMs), ligands and interaction partners, and to study conformations and soluble oligomerization intermediates. You will also collaborate with a senior cryo-EM scientist for structural analysis of aggregates.
You should have a PhD in Biophysics, Chemistry, Structural Biology, Biochemistry or a closely allied discipline with hands-on experience for example in Scanning Probe Microscopies or other types of bionanotechnology, and/or Mass Spectrometry workflows such as proteomics or structural MS.
The University of Leeds and the Faculty of Biological Sciences are committed to providing equal opportunities for all and offer a range of family friendly policies. The University is a charter member of Athena SWAN (the national body that promotes gender equality in higher education), and the Faculty of Biological Sciences was awarded a Silver award in 2020. We are proud to be an inclusive Faculty that values all staff, and are happy to consider job share applications and requests for flexible working arrangements from our employees. Our Athena SWAN webpage provides more information.
To explore the post further or for any queries you may have, please contact:
Neil Ranson, Professor of Structural Molecular Biology
Tel: +44 (0) 113 343 7065 | Email: [email protected] | Twitter: @naranson
Paolo Actis, Associate Professor in Bionanotechnology
Tel: +44 (0)113 343 34625 | Email: [email protected] | Twitter: @paoloactis
Frank Sobott, Chair in Biomolecular Mass Spectrometry
Tel: +44 (0)113 343 2576 | Email: [email protected] | Twitter: @franksobott
|Location:||Leeds - Main Campus|
|Faculty/Service:||Faculty of Biological Sciences|
|School/Institute:||School of Molecular & Cellular Biology / Astbury Centre for Structural Molecular Biology|
|Salary:||£34,304 to £40,927 p.a.|
|Post Type:||Full Time|
|Contract Type:||Fixed Term (for 3 years due to external funding)|
|Release Date:||Wednesday 20 July 2022|
|Closing Date:||Friday 26 August 2022|
The University community is made up of a wide range of people with diverse backgrounds and circumstances, which we value and regard as a great asset. As part of our continued commitment to equality and inclusion, we strive to create an environment where everyone can reach their full potential and have a real opportunity to participate in and contribute to our activities.