Date Posted: Jul 16, 2019
Application Deadline: Open Until Filled
The Nolen laboratory at the University of Oregon (https://blogs.uoregon.edu/nolenlab/) seeks a highly motivated postdoctoral researcher to join our lab full time on a flexible start date. Our interest is in understanding the molecular basis for the regulation of the actin cytoskeleton. We apply a broad range of techniques to understand the structure-function relationships of actin regulatory proteins and how their molecular mechanisms dictate cellular function. Example of current projects include:
1. Dissection of the mechanism and regulation of the WISH/DIP/SPIN90 (WDS) family proteins.
Approaches used in this project include (but are not limited to) single molecule TIRF microscopy and ensemble methods to determine biochemical mechanism of WDS proteins, x-ray crystallography to determine high resolution structures of WDS family proteins bound to Arp2/3 complex, yeast genetics and live cell imaging combined with structure-function analyses to understand how WDS proteins function in assembly of branched actin networks during endocytosis, and biochemical reconstitution of branched actin network initiation and propagation.
2. Mechanism of Arp2/3 complex regulation.
Approaches used in this project include (but are not limited to) x-ray or electron microscopy to determine high-resolution structures of Arp2/3 complex with bound regulators, single molecule TIRF microscopy and ensemble methods to determine mechanisms of branching nucleation, development of new biochemical/biophysical/optogenetic tools to explore the conformational and kinetic pathway to branching nucleation, and structure-based mutational analysis to test biochemical mechanisms in vitro and in yeast.
Successful candidates will have a Ph.D. in biochemistry, biophysics, structural or cellular biology or a related field, a published record of accomplishment, and good written and oral communication skills. This position is subject to renewal annually, but can be renewed for up to five years.
Eugene, Oregon is an ideal place to work and live, and is an outdoor enthusiast’s paradise. In addition to a mild climate with access to the ocean and mountains, Eugene has affordable housing, bike-friendly streets, and a strong community feel. The University of Oregon is an Equal Opportunity/Affirmative Action Institution committed to cultural diversity and compliance with the Americans with Disabilities Act.
For further information, please see:
Rodnick-Smith, M., Luan, Q., Liu, S.-L., & Nolen, B. J. (2016). Role and structural mechanism of WASP-triggered conformational changes in branched actin filament nucleation by Arp2/3 complex. Proceedings of the National Academy of Sciences, 113(27), E3834–E3843. http://doi.org/10.1073/pnas.1517798113
Wagner, A. R., Luan, Q., Liu, S.-L., & Nolen, B. J. (2013). Dip1 Defines a Class of Arp2/3 Complex Activators that Function without Preformed Actin Filaments. Current Biology : CB, 23(20), 1990–8. doi:10.1016/j.cub.2013.08.029
Helgeson, L. a, & Nolen, B. J. (2013). Mechanism of synergistic activation of Arp2/3 complex by cortactin and N-WASP. eLife, 2, e00884. doi:10.7554/eLife.00884
Luan, Q., & Nolen, B. J. (2013). Structural basis for regulation of Arp2/3 complex by GMF. Nature Structural & Molecular Biology, 20(9), 1062–8. doi:10.1038/nsmb.2628
To apply, please upload a cover letter, curriculum vitae, and contact information for three references to http://careers.uoregon.edu/cw/en-us/job/524303/postdoctoral-scholar. The position will remain open until filled.