Date Posted: Sep 8, 2018
Application Deadline: Open Until Filled
The Washington University School of Medicine has a Postdoctoral Research Associate position available in Dr. Carl DeSelm’s group to work on CAR T-cells for solid tumors.
Our ultimate goal is to develop new cures for cancer patients by modifying immune cells. Chimeric Antigen Receptor (CAR) T-cell therapy has dramatically improved the long-term survival of relapsed/refractory leukemia patients, demonstrating that we can achieve new levels of cure for aggressive and otherwise terminal cancers using this strategy. We are specifically working to extend this technology to a wider range of cancers. The laboratory focuses on designing and cloning novel CARs, introducing them into T cells and other immune cells, and testing their efficacy against solid tumors (currently pancreatic cancer, breast cancer, glioblastoma, and prostate cancer).
The position offers an excellent opportunity to conduct research in a supportive and stimulating environment, closely collaborating with leading Immunologists and physicians. Washington University is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, age, sex, sexual orientation, gender identity or expression, national origin, genetic information, disability, or protected veteran status.
MD and/or PhD, preferably relating to Immunology or Molecular Biology, with significant bench laboratory experience
• Experience with mouse models of cancer
• Mouse T cell isolation
• Retroviral production and transduction of cell lines
• Cell culture – primary cells and cell lines, meticulous sterile technique
• In vivo bioluminescent imaging
• Cytotoxic T lymphocyte (CTL) assays
• Immune phenotyping of tumor tissues, using immunology techniques including multi-color flow cytometry
• Knockout of genes in cancer cells and T cells using CRISPR
• CRISPR library experience
• Experience culturing macrophages or dendritic cells
• Isolation of immune cells from tumor samples
• Experience with live cell imaging