Date Posted: Jan 22, 2018
Application Deadline: Open Until Filled
We are looking for two experimentalists to join our projects funded by the French National Research Agency, the French National Cancer Institute and the Ligue contre le cancer. The research activities of the lab are centered on the study of DNA double-strand break (DSB) repair and on how these mechanisms are involved in genome integrity maintenance, cancer development and treatment.
The projects are focused on the study of the Non-Homologous End Joining (NHEJ) mechanism; the primary pathway used by human cells to repair DSBs, including DSBs induced by ionizing radiation used for cancer radiotherapy, and those induced during rearrangements of antigen and T-cell receptor genes in developing lymphocytes. Alternatively, we have projects focused on understanding Homologous Recombination mechanisms involving the ATP-dependent RAD51 and DMC1 recombinases and their associated modulators, as well as the RAD52 protein and the MRE11/RAD50/NBS1 complex.
To achieve quantitative understanding of the molecular mechanisms underlying DSB repair processes, our team combines various experimental approaches using biochemistry, molecular and cell biology, single-molecule micromanipulation (by optical tweezers), and single-molecule imaging in individual cells (by single-particle-tracking and super-resolution microscopy). In the lab, we have purified most of the human proteins involved in these DSB repair pathways (including Ku, XRCC4, XLF, LIG4, PAXX, DNA-PKcs, RAD51, DMC1, RAD52 and MRN). Furthermore, we have developed in vitro bulk and single-molecule assays for our studies using fluorescently labeled proteins and we are also editing the genome of different cell lines using CRISPR/Cas9 for studies in living cells. The single-molecule instrumentation is fully operational and running. We, thus, expect the candidates to be in a privileged position in terms of scientific productivity and availability of resources and support in the different aspects of the projects.
Depending on the interests, the motivation, and the skills of the candidate, projects can be developed either in vitro (at bulk or single-molecule level) or in cells. Background in biophysics is desired for one of the positions.
Recent publications of the team include:
- Normanno et al., eLife 6: e22900 (2017)
- Brouwer et al., Cell Reports 18, 2845 (2017)
- Tadi et al., Cell Reports 17, 541 (2016)
- Brouwer et al., Nature 535, 566 (2016)
- Roy et al., Mol. Cell. Biol. 35, 3017 (2015)
- Candelli et al., PNAS 111, 15090 (2014)
Interested candidates with at least one first author publication in a reputable journal should contact Mauro Modesti (email@example.com) providing a CV, a motivation letter, and at least two recommendation letters written by persons in the position of assessing the research capabilities and skills of the candidate.